Hexapeptide-3
Early humanSynthetic hexapeptide
Research material. Not for human or animal consumption.
Research overview
Hexapeptide-3, usually supplied as Acetyl Hexapeptide-3 or Argireline, is the six residue peptide Ac-Glu-Glu-Met-Gln-Arg-Arg-NH2. It mimics the N-terminal end of SNAP-25, a protein in the SNARE complex that releases acetylcholine at the nerve-muscle junction. By competing for a place in that complex it was proposed to reduce muscle contraction behind expression lines, a topical idea loosely compared with botulinum toxin. Human data come from small formulation studies, including a 24-participant, 60-day trial.
Limited, exploratory, early phase or small human studies have been identified.
Questions about Hexapeptide-3
PRODUCT QUESTIONSWhat is Hexapeptide-3?
Hexapeptide 3 is an acetylated and amidated six residue peptide whose sequence copies the N terminal region of SNAP 25. It is a cosmetic ingredient and is not related in structure to botulinum toxin.
What research has been published on Hexapeptide-3?
Laboratory work showed the peptide can interfere with SNARE complex assembly and reduce catecholamine release in cultured cells. Clinical evidence is limited to short studies of finished creams, such as a 24 participant, 60 day trial, measuring wrinkle depth with skin imaging. Whether enough peptide reaches facial muscle through intact skin is still debated.
What batch documentation should accompany Hexapeptide-3?
Hexapeptide-3: Ask for the COA and analytical reports for the batch being quoted. Check that mass spectrometry confirms both the acetyl cap and the C terminal amide, and that HPLC reports the methionine sulfoxide variant, the commonest degradation product.
How should Hexapeptide-3 storage conditions be assessed?
Hexapeptide-3 contains methionine, which oxidises in air and light. Keep the lyophilised powder sealed at −20°C or 2°C to 8°C, protect it from light and limit headspace oxygen in opened vials.