Thymosin β4 (1–4)
PreclinicalEndogenous peptide fragment
Research material. Not for human or animal consumption.
Research overview
Thymosin β4 (1-4) is N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), the first four residues of thymosin β4. It is released from the parent protein by prolyl oligopeptidase and broken down by angiotensin-converting enzyme (ACE), so ACE inhibitors raise its levels. Ac-SDKP was first described as an inhibitor of haematopoietic stem cell proliferation (studied as goralatide) and later as an anti-fibrotic and anti-inflammatory peptide in heart, kidney and lung models. Its evidence should not be confused with that for full-length thymosin β4.
Laboratory, computational, ex vivo or animal model research is the main evidence base.
Questions about Thymosin β4 (1–4)
PRODUCT QUESTIONSWhat is Thymosin β4 (1–4)?
Thymosin β4 (1–4) is the acetylated tetrapeptide serine, aspartic acid, lysine, proline, also called Ac SDKP or goralatide. It is a naturally occurring fragment of the 43 residue thymosin β4 protein.
What research has been published on Thymosin β4 (1–4)?
Early work studied Ac SDKP as a natural regulator that keeps haematopoietic stem cells resting, including trials as a marrow protecting agent. Later research in rodents linked it to reduced collagen deposition and inflammation in models of hypertension, cardiac fibrosis and kidney disease, and to the protective effects of ACE inhibitors.
What batch documentation should accompany Thymosin β4 (1–4)?
Thymosin β4 (1–4): Ask for the COA and analytical reports for the batch being quoted. Confirm the acetylated mass by mass spectrometry, check that HPLC resolves the deacetylated peptide, and make sure the certificate names the fragment rather than full length thymosin β4.
How should Thymosin β4 (1–4) storage conditions be assessed?
Store the lyophilised tetrapeptide sealed at −20°C, dry and away from light. The aspartic acid next to lysine can slowly rearrange in solution, so make up solutions shortly before use.